@article{oai:nvlu.repo.nii.ac.jp:00000163,
 author = {KOBAYASHSI, Jun and TERADA, Hiroshi and IKEDA, Keiichi and SUGIYAMA, Hideo},
 issue = {60},
 journal = {日本獣医生命科学大学研究報告},
 month = {},
 note = {The purpose of this study was to develop a method for determining the adhesive ability of cultured cells. The method differs from the conventional methods, and considers the blood stream on the cell adhesion. Also, to keep the cell suspension to be uniform, the procedure was conducted by rotary shaking (120 rpm) the  1X10⁶ cultured  cells/10 mL  in a 10 cm diameter laboratory dish at 37℃ using a bio-shaker. Measurements of the number of cells remaining in the supernatant were performed over time by using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide) method. The adhesive ability of the cells was determined as the time when 50% of the cells had been floating in the supernatant. Four cell lines (8505C, human thyroid carcinoma ; 8305C, human thyroid carcinoma ; A431, human epidermoid carcinoma ; CHO-K1, Chinese hamster ovary) were distinguishable for the adhesive ability (113.1, 131.6, 33.2 and 221.1 min, respectively). For example, by trypsin treatment to harvest nearly all the cell from the laboratory dish bottoms, general reaction times (8505C, 3 min ; 8304/C, 1.5 min ; A431, 7 min ; CHO-K1, 1.5 min) are reverse order above. These results do not contradict the conventional methods. This method is simple and does not need skilled techniques unlike the conventional methods.},
 pages = {67--72},
 title = {Development of Dynamic Cell Adhesion　Assay under Rotary Shaking},
 year = {2011}
}