{"created":"2023-06-19T09:04:47.419819+00:00","id":162,"links":{},"metadata":{"_buckets":{"deposit":"d993892c-13c3-42ed-86cb-56a818ab772d"},"_deposit":{"created_by":14,"id":"162","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"162"},"status":"published"},"_oai":{"id":"oai:nvlu.repo.nii.ac.jp:00000162","sets":["1:18"]},"author_link":["201","130","202","199","197","200","196","198"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"60","bibliographicPageEnd":"66","bibliographicPageStart":"58","bibliographic_titles":[{"bibliographic_title":"日本獣医背名科学大学研究報告"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"今回我々は，医学生物学研究の研究用ツールの開発を日指して，Green Fluorescent Protein (GFP)トランスジェニック(Tg) マウスの作製を行った。 BDF1雌マウスから採取した250個の受精卵にCAGプロモーターの下流にGFP遺伝子を連結した導入遺伝子をマイクロインジェクションし，1 匹の GFP Tg マウスの作出に成功した。 発生の各時期におけるGFP発現プロファイルを蛍光顕微鏡， 非侵襲的な in vivo imaging system (IVIS) およびフローサイトメトリーを用いて解析した。 GFPTg マウスの遺伝子型を Real time PCR法を用いて判定した。 GFPの発現は， 発生のいずれの時期においても全身性に強く認められた。 末梢白血球の GFP 陽性率は90% 以上と高率であった。 Real time PCR 法は， 導入遺伝子の遺伝子型を明確に判定することが出来た。本研究により全身性に GFP を強く発現する GFP Tg マウスの作出に成功した。系統化に成功した日獣大オリジナルの GFP Tg マウスは， 発生学あるいは幹細胞を利用した再生医学研究等，幅広く医学生物学研究に有用な研究ツールとなる。\n","subitem_description_type":"Abstract"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18827314","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"丸山, 基世"}],"nameIdentifiers":[{"nameIdentifier":"196","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤澤, 正彦"}],"nameIdentifiers":[{"nameIdentifier":"197","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10508873","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10508873"}]},{"creatorNames":[{"creatorName":"中垣, 和英"}],"nameIdentifiers":[{"nameIdentifier":"198","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"羽山, 伸一"}],"nameIdentifiers":[{"nameIdentifier":"199","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80183565","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80183565"}]},{"creatorNames":[{"creatorName":"福田, 孝一"}],"nameIdentifiers":[{"nameIdentifier":"200","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三好, 一郎"}],"nameIdentifiers":[{"nameIdentifier":"201","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 実"}],"nameIdentifiers":[{"nameIdentifier":"130","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"袴田, 陽二"}],"nameIdentifiers":[{"nameIdentifier":"202","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00218380 ","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00218380 "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-07-15"}],"displaytype":"simple","filename":"058 _NO.60-9.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://nvlu.repo.nii.ac.jp/record/162/files/058 _NO.60-9.pdf"},"version_id":"fe7179e0-1baf-467a-a14f-f3cb581e6493"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"トランスジェニックマウス","subitem_subject_scheme":"Other"},{"subitem_subject":"GFP","subitem_subject_scheme":"Other"},{"subitem_subject":"in  vivoイメージングシステム","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"日獣大オリジナルのGFPトランスジェニックマウスの作製および特性解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"日獣大オリジナルのGFPトランスジェニックマウスの作製および特性解析"},{"subitem_title":"Establishment of GFP transgenic mice for biomedical research","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"14","path":["18"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-07-15"},"publish_date":"2016-07-15","publish_status":"0","recid":"162","relation_version_is_last":true,"title":["日獣大オリジナルのGFPトランスジェニックマウスの作製および特性解析"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-19T09:10:39.674613+00:00"}