{"created":"2023-06-19T09:04:47.285697+00:00","id":159,"links":{},"metadata":{"_buckets":{"deposit":"a488e18c-4441-468e-8a74-1d735124a094"},"_deposit":{"created_by":14,"id":"159","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"159"},"status":"published"},"_oai":{"id":"oai:nvlu.repo.nii.ac.jp:00000159","sets":["1:18"]},"author_link":["105"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"60","bibliographicPageEnd":"49","bibliographicPageStart":"39","bibliographic_titles":[{"bibliographic_title":"日本獣医生命科学大学研究報告"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"細胞増殖/毒性測定用試薬 MTT(3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide)の生細胞数計測に影響を与える因子について調べた。その結果，以下の事が新たに確認された :1)牛胎児血清(FBS)濃度や培地の組成が，反応に影響を与える。2)MTT自身は保存中に培地成分と共存すると分解され易く，特に室温でFBSと混合されるとー日で著明な分解が起きる。3)接着性細胞は接着の有無により反応性が変化する。4)金属の共存はキレー卜効果によって干渉される。5)反応容器によって誤差を生じることがある。\n","subitem_description_type":"Abstract"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18827314","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 淳"}],"nameIdentifiers":[{"nameIdentifier":"105","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-07-15"}],"displaytype":"simple","filename":"039 _NO.60-6.pdf","filesize":[{"value":"886.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://nvlu.repo.nii.ac.jp/record/159/files/039 _NO.60-6.pdf"},"version_id":"9c957f64-8ebb-4361-8c1a-f34b7ea0dc3b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MTT","subitem_subject_scheme":"Other"},{"subitem_subject":"生細胞数計数","subitem_subject_scheme":"Other"},{"subitem_subject":"妨害","subitem_subject_scheme":"Other"},{"subitem_subject":"酸化還元","subitem_subject_scheme":"Other"},{"subitem_subject":"WST-1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"MTTを用いた生細胞数計測法に影響を与える因子について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"MTTを用いた生細胞数計測法に影響を与える因子について"}]},"item_type_id":"10002","owner":"14","path":["18"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-07-15"},"publish_date":"2016-07-15","publish_status":"0","recid":"159","relation_version_is_last":true,"title":["MTTを用いた生細胞数計測法に影響を与える因子について"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-19T09:10:51.673314+00:00"}