{"created":"2023-06-19T09:04:44.329298+00:00","id":101,"links":{},"metadata":{"_buckets":{"deposit":"f2f9563d-4eec-4dfe-91f8-8e88768dfc3b"},"_deposit":{"created_by":14,"id":"101","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"101"},"status":"published"},"_oai":{"id":"oai:nvlu.repo.nii.ac.jp:00000101","sets":["1:21"]},"author_link":["140","143","107","106","141","144","142","105","145","139"],"item_10002_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"フォトダイオードアレイ検出HPLC を用いた微分クロマトグラフ法によるカフェインの定量"}]},"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"57","bibliographicPageStart":"48","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"日本獣医生命科学大学研究報告"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We assessed caffeine levels in bathwater and diluted urine samples by derivative processing of chromatograms obtained through the use of HPLC with photodiode array detection. Chromatograms were differentiated on the basis of d（Abs）/d（min）．The peak position of the target analyte, caffeine, was observed to shift depending on the derivative order applied, but was constant when applying the same derivative order, such that retention times were constant even after differentiation. When the method detection limit was examined using standard solutions, the varying absorption of acetonitrile during a linear gradient elution from 1 to 100 % acetonitrile was shown to have an effect, such that the linearity of the peak height deteriorated if caffeine was present at low concentrations. Recovery of caffeine from real samples was optimized in the case of both linear gradient elution and isocratic elution（ 10 % acetonitrile）when applying the second derivative and using a detection wavelength of 259 nm. Interestingly, this does not correspond to the maximum absorption of caffeine. Thus the optimum wavelength for use with this differential chromatographic method is one that is not affected by the absorbance of the eluent and at which the target analyte shows significant absorbance, even if it is not the maximum absorbance. Such conditions were found to be necessary for the high-sensitivity measurement of caffeine. It is believed that this method is applicable to the HPLC analysis of compounds other than caffeine and this technique is expected to be effective when applied to an automated analyzer and should be helpful to analysts unfamiliar with HPLC.\n","subitem_description_type":"Abstract"},{"subitem_description":"フォトダイオードアレイ検出HPLC を用いた微分クロマトグラフ法による希釈尿あるいは風呂水からのカフェインの回収について検討した。通常のクロマトグラムを微分（d（Abs）/d（min））し，微分クロマトグラムを得る。目的の化合物のピーク位置は，微分次数によるずれるが，同じ微分次数で比較すると一定しており，保持時間による定性能は微分後も維持されていた。標準溶液を用いた定量下限値は，リニアグラジエント溶出（1 → 100 ％アセトニトリル）では溶離液として用いたアセトニトリルの吸収が影響を与え，カフェインが低濃度の場合にはピーク高さとの直線性が悪化した。実試料からのカフェインの回収は，波長259 nm，2 次微分実行時にリニアグラジエント溶出，アイソクラティック溶出（10 ％アセトニトリル）共最適であった。この条件は必ずしもカフェインが極大吸収を持つ波長，微分次数ではない。本結果は微分クロマトグラフ法での検出条件として，1）溶離液吸収の影響を受け難い波長を選択すること，2）目的物質であるカフェインの吸光度が極大でなくてもある程度大きいことが高感度測定に必要であることを示す。今回の結果はカフェインのみならず，他の化合物をHPLC 分析する場合，特にHPLC に不慣れな使用者が条件検討する場合や自動分析装置に応用する場合にも利用可能な知見であると考えられる。","subitem_description_type":"Abstract"}]},"item_10002_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"105","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小林, 淳"}]},{"nameIdentifiers":[{"nameIdentifier":"106","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090453597","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}],"names":[{"name":"池田, 啓一"}]},{"nameIdentifiers":[{"nameIdentifier":"107","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010260267","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}],"names":[{"name":"寺田, 宙"}]},{"nameIdentifiers":[{"nameIdentifier":"145","nameIdentifierScheme":"WEKO"},{"nameIdentifier":" \t50409257 ","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm= \t50409257 "}],"names":[{"name":"望月, 眞理子"}]},{"nameIdentifiers":[{"nameIdentifier":"139","nameIdentifierScheme":"WEKO"}],"names":[{"name":"杉山, 英男"}]}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18827314","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KOBAYASHI, Jun"}],"nameIdentifiers":[{"nameIdentifier":"140","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IKEDA, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"141","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TERADA, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"142","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MOCHIZUKI, Mariko"}],"nameIdentifiers":[{"nameIdentifier":"143","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SUGIYAMA, Hideo"}],"nameIdentifiers":[{"nameIdentifier":"144","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-06-10"}],"displaytype":"simple","filename":"小林　淳_2.pdf","filesize":[{"value":"4.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PDF","url":"https://nvlu.repo.nii.ac.jp/record/101/files/小林　淳_2.pdf"},"version_id":"4f0115e4-78ca-45dd-8b43-8195cf3138c9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"derivative chromatographic method","subitem_subject_scheme":"Other"},{"subitem_subject":"caffeine","subitem_subject_scheme":"Other"},{"subitem_subject":"urine","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"HPLC Determination of Caffeine Using a Photodiode Array Detector and Applying a Derivative Processing to Chromatograms","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"HPLC Determination of Caffeine Using a Photodiode Array Detector and Applying a Derivative Processing to Chromatograms"}]},"item_type_id":"10002","owner":"14","path":["21"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-06-10"},"publish_date":"2016-06-10","publish_status":"0","recid":"101","relation_version_is_last":true,"title":["HPLC Determination of Caffeine Using a Photodiode Array Detector and Applying a Derivative Processing to Chromatograms"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-06-19T09:10:25.834428+00:00"}