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Development of Dynamic Cell Adhesion Assay under Rotary Shaking
https://nvlu.repo.nii.ac.jp/records/163
https://nvlu.repo.nii.ac.jp/records/1636a471eaa-637a-4c7e-85fa-42d58a119f5e
名前 / ファイル | ライセンス | アクション |
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PDF (302.7 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-07-15 | |||||
タイトル | ||||||
タイトル | Development of Dynamic Cell Adhesion Assay under Rotary Shaking | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adhesion assay | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cultured cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Rotary shaking | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | 回転振盪下での動的細胞接着能測定法の開発 | |||||
著者 |
KOBAYASHSI, Jun
× KOBAYASHSI, Jun× TERADA, Hiroshi× IKEDA, Keiichi× SUGIYAMA, Hideo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The purpose of this study was to develop a method for determining the adhesive ability of cultured cells. The method differs from the conventional methods, and considers the blood stream on the cell adhesion. Also, to keep the cell suspension to be uniform, the procedure was conducted by rotary shaking (120 rpm) the 1X10⁶ cultured cells/10 mL in a 10 cm diameter laboratory dish at 37℃ using a bio-shaker. Measurements of the number of cells remaining in the supernatant were performed over time by using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide) method. The adhesive ability of the cells was determined as the time when 50% of the cells had been floating in the supernatant. Four cell lines (8505C, human thyroid carcinoma ; 8305C, human thyroid carcinoma ; A431, human epidermoid carcinoma ; CHO-K1, Chinese hamster ovary) were distinguishable for the adhesive ability (113.1, 131.6, 33.2 and 221.1 min, respectively). For example, by trypsin treatment to harvest nearly all the cell from the laboratory dish bottoms, general reaction times (8505C, 3 min ; 8304/C, 1.5 min ; A431, 7 min ; CHO-K1, 1.5 min) are reverse order above. These results do not contradict the conventional methods. This method is simple and does not need skilled techniques unlike the conventional methods. | |||||
書誌情報 |
日本獣医生命科学大学研究報告 号 60, p. 67-72, 発行日 2011 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18827314 |