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Simultaneous Inactivation of the p16, p15, and p14 Genes Encoding Cyclin-Dependent Kinase Inhibitors in Canine T-lymphoid tumor cells
https://nvlu.repo.nii.ac.jp/records/117
https://nvlu.repo.nii.ac.jp/records/11798fbc2aa-167e-4db4-9584-f088413030be
名前 / ファイル | ライセンス | アクション |
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PDF (2.2 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-06-16 | |||||
タイトル | ||||||
タイトル | Simultaneous Inactivation of the p16, p15, and p14 Genes Encoding Cyclin-Dependent Kinase Inhibitors in Canine T-lymphoid tumor cells | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | canine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | lymphoid tumor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | p16 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
藤原, 亜紀
× 藤原, 亜紀 |
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著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 158 | |||||
姓名 | IGARASHI, Aki | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The p16, p15, and p14 genes are widely known as tumor suppressor genes in human medicine. Although a large number of genetic and epigenetic aberrations in these genes have been reported in human malignancies, canine malignancies have not been well analyzed on the aberrations of these genes. In this study, the full-length complementary DNA (cDNA) of the canine p16 gene was cloned using the 5′ and 3′ rapid amplification of cDNA ends methods. Based on the sequence data, primers specific for p16, p15, and p14 were designed. Using these primers, the expression of p16, p15, and p14 mRNAs could be individually evaluated by reverse transcriptase polymerase chain reaction. Genomic aberrations were also examined using genomic polymerase chain reaction. Two of the 6 canine lymphoid tumor cell lines did not express detectable levels of p16, p15, and p14 mRNAs, and wide-ranging deletions in the p15-p14-p16 genomic locus were suspected. Wide-ranging deletions were also speculated in 2 of 14 dogs with T-cell lymphoid tumors. On the other hand, similar failure of amplification suggesting wide-ranging deletions were not observed in any of the 14 dogs with B-cell lymphoma. Deletion of the p15-p14-p16 genomic locus could be one of the molecular aberrations in canine lymphoid tumor cells. | |||||
書誌情報 |
日本獣医生命科学大学研究報告 巻 62, p. 31-42, 発行日 2013-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18827314 |